The Biobrio 10(2 & 3), 2023

Screening of Lipolytic activity containing isolates of Lactobacillus sps. isolated from cow milk

Swikriti Kumari & Arun Kumar

ABSTRACT:

The present study was conducted to determine lipolytic activity of isolates of Lactobacilli isolated from cow milk. Altogether, seven isolates were isolated belonging to Lactobacillus brevis and Lactobacillus fermentum. Isolate no. Lb1, Lb2, Lb3 and Lb4 belong to Lactobacillus brevis and isolate no. Lf1, Lf2 and Lf3 belongs to Lactobacillus fermentum. All isolates were gram positive, catalase, oxidase and indole negative, Glucose lactose and mannitol positive, sucrose fructose and raffinose negative. All isolates showed positive KIA test. Lipase production was maximum at a time interval of 15 minutes. Among L. brevis, isolate no. Lb2 showed maximum lipase production (115 unit) and minimum lipase production was observed in isolate no. Lb4 (103 unit). Among isolates of L. fermentum, maximum lipase production was observed in isolate no. Lf3 (130 unit) and minimum lipase production was observed in isolate no. Lf2 (120 unit).

Keywords:    

Lipase activity, Isolates, Lactobaciili, Food and Pharmaceutical industries.

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