Screening of Lypolytic activity of Lactobacilli isolates from cow milk

Enzyme Lipase catalyses hydrolysis of triglycerides to produce glycerol and fatty acid. This enzyme have immense importance in several industries like dairy, food and pharmaceutical industries. Lipase of Lactobacilli is more important. In the present study, Lipase activity of some isolates of Lactobacilli isolated from cow milk was examined. Altogether, 13 isolates belonging to 3 species of Lactobacilli were examined. Out of 13 isolates, 6 belongs to Lactobacillus casei, 4 belongs to Lactobacillus acidophilus and 3 belongs to Lactobacillus fermentum. The lipase activity was maximum at a time interval of 15 minutes and it decreased at higher and lower time interval. Among isolates, maximum lipase production was observed in isolate No. LF3 of L. fermentum (120 unit) at a time interval of 15 minutes and minimum lipase production was observed in isolate No. LA1 of L. acidophilus (112 unit) at a time interval of 15 minutes.