The Biobrio 5(3 & 4), 2018
Callus induction from Nodal and Internodal explant of Mucuna pruriens
(L.) DC. - A potent medicinal plant
Moti Lal Srivastva and Md. Sarfaraz Ahmad
ABSTRACT:
A rapid and efficient in vitro multiplication of Mucuna pruriens (L.) DC., a valuable and potent
medicinal plant was achieved by culturing internodal explants on MS medium containing
different types of hormone 6-benzyl aminopurine (BAP), kinetin (Kn) and isopentenyl
adenine (2-iP)NAA, 2,4 -D. Result are obtained after 21days of culture. The optimal
regeneration observed at different concentra??on with MS mediumin various concentration
-1 and combination of 2,4-Dat an optimal concentration of 4.5 mgl and BAP at an optimal
-1 concentration at 3.5 mgl .
Keywords: Callus induction, nodal, internodal, Mucuna pruriens
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