The Biobrio 5(3 & 4), 2018

Callus induction from Nodal and Internodal explant of Mucuna pruriens (L.) DC. - A potent medicinal plant

Moti Lal Srivastva and Md. Sarfaraz Ahmad

ABSTRACT:

A rapid and efficient in vitro multiplication of Mucuna pruriens (L.) DC., a valuable and potent medicinal plant was achieved by culturing internodal explants on MS medium containing different types of hormone 6-benzyl aminopurine (BAP), kinetin (Kn) and isopentenyl adenine (2-iP)NAA, 2,4 -D. Result are obtained after 21days of culture. The optimal regeneration observed at different concentra??on with MS mediumin various concentration -1 and combination of 2,4-Dat an optimal concentration of 4.5 mgl and BAP at an optimal -1 concentration at 3.5 mgl .

Keywords:    Callus induction, nodal, internodal, Mucuna pruriens