ISSN: 2393-9508
e-ISSN: 2582-4902

The Biobrio 9(3 & 4), 2022

Leaf area index estimation using grid counting method

Sneha Sahay, Prasanjit Mukherjee & Jyoti Kumar


The easy, economic, and precise estimate of leaf surface area has been a concern to plant scientists for a long time. Leaf area estimate is valuable in studies of plant nutrition, plant competition, plant-soil-water relations, plant protection measures, respiration, light reflectance, and heat transfer in plants. To count the number of square cm., a simple approach is to construct unit squares within the interested area using the graph paper that has 1-centimeter square grid lines can be used the millimeter graph paper method described in this paper was used to estimate individual leaf area of four plant species. The leaf area measurement of Duranta erecta, Phyla nodiflora, Stachytarpheta jamaicensis and Lippia alba are 16.6 ± 2.796, 10.0 ± 1.632, 12.6 ± 1.349 and 59.2 ± 18.599 respectively. The leaf area is maximum in Lippia alba and minimum in Phyla nodiflora.


Leaf area, Leaf area measurement, graph paper

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